6^th Global Veterinary Summit November 14-16, 2016 Atlanta, USA

Biography:

Daniel Alfonso Gonzalez Acuna has completed his PhD in the Institute of Wildlife at the Veterinary Medicine Superior School of Hannover, Germany. He is a Veterinarian, specialized in diseases of wild birds, mainly on the relation with their parasites and also a full Professor in the University of Concepcion, Chile. He has developed different research projects about parasites in wild birds. He has published about 150 papers in national and international journals

Abstract:

Ticks (Ixodoidea: Ixodidae, Argasidae) are obligatory hematophagous ectoparasites of vertebrates in some or all postembryonic stages. Several species are vectors of agents that may cause disease and even kill their vertebrate hosts; ticks may also cause dermatoses and loss of blood and they can inoculate toxins. Globally, the superfamily Ixodoidea contains the families Argasidae (208 species), Ixodidae (722 species) and the African Nuttalliellidae (monotipic). In the Neotropical region, a total of 200 tick species have been documented; 84 soft ticks and 116 hard ticks. In Chile, before 2006, approximately 20 tick species were known to inhabit Chile. In the last 10 years, the biogeographic and taxonomic knowledge has increased markedly due to two research projects by our group that allowed us to detect new findings and make new descriptions of ticks in Chile. We have collected during 7 years (360 days of field work) ticks of different hosts and environments in the 14 eco-regions of Chile, including the Antarctic Territory. We have developed the biological cycles under laboratory conditions, determined new species and new records of ticks for Chile and recently also, we have been researching the role of ticks as reservoirs of pathogens. The knowledge of Chilean ticks is increasing, being as we describe 31 ticks present in Chile, five of them new species. We have developed the complete biological cycles of four soft ticks. With respect to tick borne diseases, some Chilean tick species are threats to human health and others to domestic animals. Ornithodoros amblus and O. spheniscus cause severe discomfort in humans; Otobius megnini can play a role in the maintenance of Coxiella burneti in nature and cause Otitis in humans; Argas neghmei may cause erythema, pruritus and skin discoloration. Rhipicephalus sanguineus has been responsible for the arthropod bites of humans in Chile and is actually the most common ticks in domestic animals in the country and its bite has been correlated with the transmission of Rickettsiae to humans and animals. Recently, we have described the new bacteria Borrelia chilensis in Ixodes sigelos and Rickettsia sp., in Amblyomma parvitarsum from different localities. The integration of this knowledge will finally allow the implementation of management measures to prevent or anticipate emergent disease transmission between animal and human populations.

Biography:

Magdi Mohamed Waheed has completed his PhD from Cairo University and Professor Degree from Cairo University, Faculty of Veterinary Medicine. He has published more than 21 papers in reputed journals and has been serving as a Reviewer of repute.

Abstract:

Forty-eight ejaculates were obtained from four Arab stallions to study the impact of three extenders (INRA Freeze^®, Tris egg-yolk and E-Z mixin) on the fertilizing capacity of frozen-thawed sperm. Semen samples were diluted using the extenders with a dilution rate of 1:1 and 1:2 and cryopreserved in 0.5 ml plastic straws. Frozen straws were thawed either at 37 °C for 30 sec or 75 °C for 7 sec. Results revealed that the percentages of progressive sperm motility, live sperm and abnormalities and characters of sperm motility like path velocity (VAP, μm/s), straight line velocity (VSL, μm/s), point to point velocity (VCL, μm/s) and lateral head displacement (ALH, μm) were significantly (P<0.01) better in the INRA Freeze^® extender after thawing at 37 °C with dilution rate 1:1 and 1:2. Sperm progressive motility after thawing at 75 °C were significantly (P<0.01) higher by using INRA freeze^® and E-Z mixin extenders at rates of dilution 1:1 and 1:2. The conception rate in 40 Arab mares using 300 million forward motile sperm per insemination was significantly (P<0.01) higher by using INRA freeze^® extender that thawed at 37 °C at dilution rate of 1:1 (50%) than 1:2 (20%). The use of two times inseminations gave significantly (P<0.01) better results (50%) than the use of one time insemination (28.57%). In conclusion, the fertilizing capacity of the frozen-thawed Arabian horse sperm was the best by using INRA freeze^® extender in a dilution rate of 1:1 and two times inseminations.

Biography:

Nguyen Thi Nga has completed her Doctor of Veterinary Medicine at Hue University of Agricultural and Forestry, Vietnam, Master of Science in the field of Animal Science at Graduate School of Agriculture and Life Science, The University of Tokyo, Japan. She is currently pursuing her PhD studying on establishment of the efficiency diagnosis method for early detecting cattle fascioliasis applicable for field research in Central Vietnam one of the hot spot of human fascioliasis in the world.

Abstract:

Fascioliasis is one of the most important zoonotic diseases in Vietnam. It has been reported that infected cattle are mainly observed in low hilly and plain areas where cattle grazing place is shared with paddy fields or water plants for human consumption and prevalence of Fasciola spp., infection in cattle are rare in sandy and salty coastal plain areas. However, recently, high prevalence of cattle fascioliasis was reported in coastal area in Thua Thien Hue Province, Vietnam. Irrigation system has been constructed in coastal area from plain area for expanding paddy field. In this study, in order to investigate the impact of the irrigation system to the expansion of cattle fascioliasis in coastal area, prevalence of cattle fascioliasis and infectivity of intermediate host snail with Fasciola spp., were compared in coastal area with or without well-established irrigation system. Total of 205 cattle fecal samples and 2573 intermediate host snails of Fasciola spp., were collected from 4 communes with well-established irrigation system connected with pure water from plain area (area A; communes, Quang Ngan, Sia Town, Vinh My and Quang Phuoc) and from 4 communes where irrigation systems are partially constructed but not functionally working (area B; communes, Phu Dien, Quang Cong, Vinh Thanh and Thuan An). Prevalence of Fasciola spp., in cattle from communes in area A was 41.3% (25.0-52.2%), which was extremely higher than the prevalence in cattle from area B that was 8% (2.0-15.4%). Infectivity of intermediate host snails, Lymnaea spp., from area A and area B were 9.8% (6.3-50.0%) and 3.4% (0-5.7%), respectively. Considering that cattle fascioliasis rare in coastal area before irrigation systems were introduced, these results suggested that Fasciola parasites are introduced to coastal plain area from endemic plain area through irrigation system. Interestingly, the density of intermediate host snails were not different between area A (29.6 snail/m²) and area B (32.5 snail/m²), indicating the habitat for snails are not dependent on the irrigation system.

Biography:

Golnaz Sharafi has completed her DVM with excellent grade from Veterinary School in Tehran University, Iran. She is currently a R&D and Lab Officer in Viromed Laboratory. She has published more than 5 papers in reputed journals and also attended several international conferences.

Abstract:

Introduction & Aim: Aspergillus fumigatus is the most frequent etiologic agent of invasive Aspergillosis in immunocompromised patients. Economic significance of Aspergillus spp., in animal health is mainly characterized by their ability in production of mycotoxins and cause disease in respiratory system of poultry. The ability of a pathogen to adapt to a high temperature environment such as that in the host body is the key factor for the pathogenesis. HSP70 family of genes and their related proteins play a pivotal role in resistance of microbial cells to stressed conditions within host body. Here, we examined relative gene expression of heat-inducible HSP70 gene in environmental and clinical (from infected ostriches) isolates of Aspergillus fumigatus.

Methods: The isolates were cultured at four temperature-points (20 °C, 30 °C, 37 °C and 42 °C) for five days, quantitative real-time PCR used to measure the relative gene expression of HSP70 gene in clinical isolates in comparison to Aspergillus fumigatus ATCC 90906 standard strain which was incubated at 25 °C for five days.

Results: During entire five days HSP70 expression level in clinical samples was higher than in environmental samples (p<0.05, Mann Whitney U test). Difference in expression level between two groups at 42 °C was reduced. Mean HSP70 expression level of five incubation days showed a slow and constantly increasing pattern by temperature elevation in both groups at 30 °C, 37 °C and 42 °C but in contrary at 20 °C both groups demonstrated a decreasing expression pattern. Temperature shift from 20 °C to 42 °C resulted in a strong HSP70 induction and up to 10 and 8.6 fold change in its expression levels at the end of fifth day of incubation in clinical and environmental groups, respectively.

Conclusion: We concluded that two temperature-points including 37 °C and 42 °C are major temperature-points inducing HSP70 expression in Aspergillus fumigatus and cause highest expression shift in both experimental groups.

Biography:

Roseline Olobatoke has completed her PhD from North-West University, South Africa and Postdoctoral studies from the same university. She is a Senior Lecturer at the College of Agriculture, Ahmadu Bello University, Nigeria. She has published more than 12 papers in reputed journals and has served as a Reviewer for journals of repute.

Abstract:

This study was conducted to evaluate the resistance of non-typhoidal Salmonella strains (NTS), isolated from broiler products in the North West Province of South Africa, to antimicrobials. A total of 60 NTS isolates recovered from raw broiler products and confirmed by PCR were evaluated for antimicrobial resistance by disk diffusion method, using a panel of ten antibiotics. The NTS isolates that were identified and used in this study include S. typhimurium, S. enteritidis and S. newport. The antimicrobials used were ampicillin (10 µg), chloramphenicol (30 µg), ciprofloxacin (5 µg), amikacin (30 µg), trimethoprim-sulfamethoxasole (25 µg), tetracycline (30 µg) cefotaxime (30 µg), meropenem (10 µg), gentamicin (10 µg) and erythromycin (15 µg). Isolates resistant to ampicillin (n=48), tetracycline (n=60) and chloramphenicol (n=12) were further screened by PCR for antibiotic resistance genes, targeting the blaTEM, tet and cat genes. All the strains tested were resistant to two or more antibiotics. All isolates were susceptible to cefotaxime, meropenem, gentamycin and amikacin whereas all were resistant to tetracycline. Resistance to trimethoprim-sulfamethoxasole, ciprofloxacin and chloramphenicol was low, being 8.3%, 13.3% and 20% respectively. Multi-drug resistance was discovered in nine S. typhimurium strains, representing 15% of the tested isolates. In addition, the blaTEM gene was identified in 15 (31.3%) of the isolates screened, whereas the tet and cat genes were expressed in 12 (20%) and 6 (50%) resistant isolates respectively. The observations of this study indicate that NTS strains isolated from broiler products are resistant to multiple antibiotics, including quinolones.

Biography:

Madhurita Gupta is currently the President of Myvets Charitable Trust & Research Centre, India. She has expertise in emergency and critical care and has worked for years in United Kingdom. She is also involved in working with the world's most threatened and endangered species and has provided wild life treatment and care to elephants, chimpanzees, hanuman langur, marmosets & exotic birds before focusing solely on veterinary consultancy and training.

Abstract:

Conflict between wildlife and humans are increasing globally with the habitat destruction and the increase in the population of tigers, elephants and leopards in India. With the aim to develop a technology for safe handling of wildlife-human conflicts and safe patrolling in the wilderness “Myvets Charitable Trust & Research Centre” developed “Wildlife Rescue Bike”. In most of the reported cases of leopard rescue during conflicts majority of forest officials gets injured and there are incidences wherein villagers also got injured during the rescue operation while going near the leopards as conflict creates fear in the mind of people and they counteract by beating the wildlife to death or by killing them directly. New technologies will revolutionize the way we handle the wildlife-human conflicts and wildlife conservation, it can be used as a effective and powerful tool for wildlife conflicts mitigation worldwide. The device has been used in India in wildlife terrain and is very effective to provide safety to the forest officials & wildlife veterinarians during the leopard-human conflicts, night patrolling and during tranquilization of wild leopards and tigers for rescue.

Biography:

Charles E Short is an Emeritus Professor of Anesthesiology and Pain Management, Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, USA. He has completed his DVM at Auburn University in 1958. He is the Founding Charter Diplomate of American College of Veterinary Anesthesiologists (1975) and Diplomate of European College of Veterinary Anesthesia and Analgesia (1996). He has been honored with Dr.h.c. (doctor honoris causa) from the Faculty of Veterinary Medicine, University of Helsinki, Finland in 2015 and received his Career Achievement Award from American College of Veterinary Anesthesia and Analgesia in the same year. He has made a total of 352 publications including 155 journal publications; textbooks, Veterinary Anesthesia, 1974; The Principles and Practice of Veterinary Anesthesia, 1987 and Animal Pain, 1991; book chapters and other scientific publications. He is the Co-Founder of International Veterinary Academy of Pain Management. He has made 447 presentations and lectures for professional groups in the United States and 293 in 45 countries.

Abstract:

The management of anesthesia in animals has progressed significantly during the last 30 years. This in part is the result of the development of new and better anesthetics and anesthetic equipment. However this alone would not be enough. It was important to understand what combination of medications, dosages and expected responses in either healthy animals or those with major health problems putting them as high risk. Because of the combined efforts of members of the American College of Veterinary Anesthesia and Analgesia, safe and effective anesthesia has now become a standard of clinical practice. There are now established guidelines. It is not only important to select the better anesthetics and dosages but also to monitor during the perioperative period and be ready to respond to undesirable responses. This includes the management of fluids, oxygen concentrations and cardiovascular needs. If these are managed as they should be, assurance of maintaining desired brain function and post anesthetic recovery can be expected. Anesthetic management does not end with the placement of the last suture. Then it is necessary to follow through during recovery to assure the return to an awake state. Next is the management of post operative pain as the animal recovers from anesthesia. Even though we now have safe and effective anesthetics, a standard of care is needed for best results.

Biography:

Mushtaq A Memon has completed his PhD in Theriogenology from University of Minnesota and Residency training at University of Illinois, Urbana-Champaign. He is a Diplomate, American College of Theriogenologists and participates in teaching and providing clinical service as a part of the Comparative Theriogenology section at Washington State University (WSU). He has received certification in Veterinary Acupuncture from Chi Institute, FL in 2008. At WSU, he coordinates Complimentary & Alternative Veterinary Medicine course. He is the author or co-author of more than 170 publications in scientific journals, conference proceedings and book chapters. He has given more than 120 presentations at international, national, state/local meetings. Internationally, he has given presentations in more than 20 countries. Before joining WSU, he taught at veterinary colleges of Oklahoma State, Louisiana State and Tufts University in MA.

Abstract:

With the increasing interest in integrative therapies in human, animal owners are seeking similar therapies for their pets. Many private veterinary practices and some veterinary teaching hospitals are offering Integrative Veterinary Medicine (IVM) modalities as an adjunct to the Western therapies. The results of a recently published retrospective analysis of 5,195 integrative patient treatment sessions suggest that acupuncture, laser therapy and hydrotherapy were utilized in more than 50% of the patients. To make IVM as an integral part of veterinary practice, efforts need to be made during veterinary education of the future veterinarians. Appropriate IVM curriculum would include basic concepts of IVM, acupuncture, manual therapies, botanical medicine, integrative nutrition and physical rehabilitation. Joint efforts between IVM-related organizations are needed to assist veterinary students in learning IVM, helping veterinary faculty to become certified in IVM modalities and to increase public awareness of the IVM benefits.

Biography:

Hamdy Rezk is currently working as a Lecturer at Department of Anatomy & Embryology, Faculty of Veterinary Medicine of Cairo University, Egypt.

Abstract:

Aim: The study was conducted to evaluate the ability of BMSC to repair the degenerated beta cells of the pancreas in induced diabetic rat model.

Material & Methods: The present study was conducted on 60 adult female Sprague Dawley rats weighing 150-200 gm, divided into three groups; the first group infused with physiological saline (normal), the 2^nd group subjected to single STZ (65 mg/kg i.p.), the 3^rd group infused with two successive doses of MSC (2.5×10⁶) in the tail vein after induction of diabetic model with interval period of 21 days). The blood glucose level estimated weekly by glucometer for one month, the oxidative stress (GSH & MDA) and the insulin gene expression evaluated at the end of the study. In addition to the histopathological alteration and immunohistochmical reaction for insulin was applied to the pancreatic tissue.

Results: The blood glucose level reduced in the treated group with time till reach its acceptable level while in the diabetic group the glucose level was significantly increased. The GSH was significantly enhanced in treated group while the MDA significantly decreased compared to diabetic model. The treated group showed increased expression of the insulin gene compared to the control positive group. The immunohistochemical analysis for insulin showing increased number and size of pancreatic islets in treated group compared to the control positive one.

Conclusion: The twice injection of BMSC can restore the normal beta cells morphology and function.

Biography:

Vishnu Sunil Jaikumar has completed his Masters in Veterinary Surgery and Radiology from College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Mannuthy, Kerala, India. He is currently working as a Research Associate in Rajiv Gandhi Centre for Biotechnology, Kerala, India. Formerly, he has worked as a Veterinary Surgeon in an institution named Animal Birth Control for Dogs (ABCD) Kochi run by Kochi Municipal Corporation, the first government run facility solely for the purpose of control of stray dog population in Kerala, India.

Abstract:

The study was conducted in twelve dogs with clinical signs of full thickness non-healing cutaneous wounds brought to the University Veterinary Hospitals of Mannuthy and Kokkalai, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, India to evaluate and compare the efficacy of bovine-collagen and bovine collagen-10% alginate sheets in canine skin wound healing. The dogs were randomly divided into two groups viz., a pre-sterilized bovine collagen-10% alginate sheet (Group-I) and a pre-sterilized bovine collagen sheet (Group-II), on wound surface after thorough surgical debridement under general anesthesia. Re-application of the dressings was carried out at 48 hours intervals for three times followed by weekly bandaging. Wound attributes were studied on the day of presentation and 7^th, 14^th and 28^th days post-application. Skin punch biopsy taken from representative samples on day 28 were subjected to Gomori’s one step trichrome staining to study the extent of collagen deposition and orientation of fibers, fibroblast proliferation and regeneration. The study revealed no significant difference in the efficacy between bovine collagen - 10% alginate and bovine collagen sheets in full thickness cutaneous wound healing in dogs. Both the collagen sheets were well tolerated by the patients with no adverse reactions and healing could be observed within 28 days with near to normal histomorphology. However, addition of 10 percent alginate in bovine collagen dressings could achieve absorption of exudates significantly early, higher rates in reduction of wound surface area, wound contraction and epithelialization.

Biography:

Mohammed Baba Ardo has completed his DVM degree from University of Maiduguri, Nigeria and Masters in Preventive Veterinary Medicine at the University of Ibadan, Nigeria. He has obtained his PhD in Veterinary Public Health (2009) from the University of Nigeria. He is the Head of Department, Animal and Range Management, Modibbo Adama University of Technology, Nigeria. He has published over 30 papers in reputed journal.

Abstract:

A cross sectional study was conducted from November, 2014 to April, 2015 on 14508 slaughtered cattle at Yola Abattoir Adamawa State on Postmortem examination. The objectives of the study were to determine the prevalence of Contagious Bovine Pleuro Pneumonia (CBPP), Bovine Fasciolosis and Bovine Tuberculosis (BTB) in slaughtered cattle according to sex and breeds. A regular visit was made to the Yola Abattoir at 6:00 AM seven times a week and this was done for six months. A total of 14508 slaughtered cattle were examined at postmortem and 3418 (23.56%) cases were recorded; CBPP 898 (6.19%), Fasciolosis 1100 (7.58%) and TB 1420 (9.79%). There was statistical significance (P<0.05) in the distribution of the diseases among the slaughtered cattle. However, according to sex out of the 3418 cases, 908 (6.26%) were male while 2510 (17.30%) were female. There was high significance difference in sex distribution of the diseases and in terms of breeds, Adamawa Gudali 1006 (6.93%), Sokoto Gudali 900 (5.51%), Red Bororo 800 (6.20%) and White Fulani 712 (4.91%). There was statistical variation (P<0.05) among the breeds. This study would serve as a valuable tool in monitoring pathological condition in the study area.

Biography:

Annytha Detha is currently working as a Lecturer of Faculty of Veterinary Medicine, Nusa Cendana University, Indonesia. She has obtained her DVM and MSc degrees from Bogor Agricultural Institute in the year 2005 and 2008 respectively. She has completed her Doctoral degree of Veterinary Public Health from the same institute in the year 2014. Her research expertise includes food hygiene, veterinary public health, zoonotic diseases, epidemiology and biosecurity. She has numerous publications in various national and international journals and has participated in many conferences and seminars.

Abstract:

The purpose of this study was to evaluate the antimicrobial activity of traditional palm wine in East Nusa Tenggara, named Sopi and Moke, through evaluation of total coliform in farms. In this research 36 swab samples were collected which are derived from four farms in the city area Kupang. 36 swab samples consisted of 16 swab samples before application Sopi and Moke and 16 samples after the application Sopi and Moke. Swab samples were analyzed with total coliform test using Violet Red Bile Agar. The research shows that total coliform after application Sopi and Moke was decreased compared to before applied Sopi and Moke. The study also proved that Sopi and Moke has the same capabilities as the commercial disinfectant named Formaldes®. Therefore Sopi and Moke could be used as a natural disinfectant on farms which can be utilized by local farmers in East Nusa Tenggara.

Biography:

Damwesh Sila Daniel has completed his Masters in Animal Production and Range Management with a distinction in Research in 2012 from Modibbo Adama University of Technology, Nigeria. He has been an Instructor in Biology at Nakam Memorial School, Panyam, Nigeria. He has published 4 papers in reputed journals, was a Member of Animal Science Association of Nigeria (ASAN) and Supervisor of International Young Inventors Competitions (IEYI, IIDC) in Indonesia, Thailand and Taiwan.

Abstract:

Biotechnology is widely used in animal production with numerous other potential applications. It can be applied for improving animal performance through better nutrition, enhanced production potential or improved health status. Plant biotechnology can produce crops which are embedded with improved nutritional value or incorporate vaccines or antibodies into feeds that will cheaply and effectively protect the animals against diseases. Transgenic manipulation of commensal gut or rumen micro-organisms has considerable potential for improving nutrition, gut development and health in animals. Accelerated growth and leaner carcasses in meat animals and increased milk production in dam cows is achieved by the administration of recombinant somatotropin (SI) and by controlling the expression of the gene with ad hoc promoters thereby preventing severe impairment of the health status of transgenic animals. The paper also unfolds some biotechnologically generated possibilities through the production of genetically modified (GO) crops, value-added traits, added-value feed stuffs (quality traits) as well as the production of dietary enzymes and production of transgenic animals for use in animal production with particular bias on poultry. Animal breeding is another application. While developed nations are leading the way, developing countries are lagging behind due to a number of factors such as lack of man power, poor infrastructure /laboratories, misplaced priorities and lack of self will by both the Government and Management of the various institutions in developing nations. A drastic adjustment and redemptive action by developing and developed nations respectively is suggested as a way forward.

Biography:

Golnaz Sharafi has completed her DVM with excellent grade from Veterinary School in Tehran University. She is currently a R&D and Lab Officer in Viromed Laboratory. She has published more than 5 papers in reputed journals and also attended several international conferences.

Abstract:

Aim: A feeding trial was conducted to investigate the effects of dietary supplementations of antibiotic, probiotic, prebiotic and synbiotic on broiler performance, histomorphologic measurements of small intestine and immune response.

Material & Method: A total number of 432, day-old broiler chicks (Ross 308) were obtained and randomly assigned to 1 of 9 dietary treatments for 6 weeks. The dietary treatments were: 1; basal diet; 2, 3; basal diet plus 400, 600 g of phosphomycin product/ton of starter and grower feeds, respectively, 4, 5; basal diet plus (150,200) g of probiotic product/ton of the starter feed and 100,150 g/ton of the grower feed, respectively, 6, 7; basal diet plus 500, 1000 g of a prebiotic product/ton of starter and grower feeds, respectively, 8 and 9; basal diet plus 1000,1250 g of synbiotic product/ton of the starter feed and 500,750 g/ton of the grower feed, respectively.

Results: Birds supplemented with the synbiotic had a greater (P<0.01) feed intake and body weight gain compared with those of others treatments. Feed conversion rate was lower in birds supplemented with all additives than in control birds (P<0.01). The carcass weight was significantly increased in feed additives compared with that of control treatment group (P<0.05). The villus height was significantly increased in feed additives compared with that of control group (P<0.01). Synbiotic treated animals showed increase (p<0.05) in antibody titers against NDV compared to those of the control groups at 28, 35 and 42 days of age.

Conclusion: The result of the present study revealed that these products had promising effects as alternatives for antibiotics in parallel to demand for elimination of growth-promoting antibiotics.