Magdi Waheed
Cairo University
Egypt
Title: The effect of dilution rate and insemination protocol on fertilizing capacity of frozen-thawed Arabian horse sperm
Biography
Biography: Magdi Waheed
Abstract
Forty-eight ejaculates were obtained from four Arab stallions to study the impact of three extenders (INRA Freeze®, Tris egg-yolk and E-Z mixin) on the fertilizing capacity of frozen-thawed sperm. Semen samples were diluted using the extenders with a dilution rate of 1:1 and 1:2 and cryopreserved in 0.5 ml plastic straws. Frozen straws were thawed either at 37 °C for 30 sec or 75 °C for 7 sec. Results revealed that the percentages of progressive sperm motility, live sperm and abnormalities and characters of sperm motility like path velocity (VAP, μm/s), straight line velocity (VSL, μm/s), point to point velocity (VCL, μm/s) and lateral head displacement (ALH, μm) were significantly (P<0.01) better in the INRA Freeze® extender after thawing at 37 °C with dilution rate 1:1 and 1:2. Sperm progressive motility after thawing at 75 °C were significantly (P<0.01) higher by using INRA freeze® and E-Z mixin extenders at rates of dilution 1:1 and 1:2. The conception rate in 40 Arab mares using 300 million forward motile sperm per insemination was significantly (P<0.01) higher by using INRA freeze® extender that thawed at 37 °C at dilution rate of 1:1 (50%) than 1:2 (20%). The use of two times inseminations gave significantly (P<0.01) better results (50%) than the use of one time insemination (28.57%). In conclusion, the fertilizing capacity of the frozen-thawed Arabian horse sperm was the best by using INRA freeze® extender in a dilution rate of 1:1 and two times inseminations.