Selvinaz Yakan
Agrı Ibrahim Cecen University, Turkey
Title: The effects of hypericum perforatum and polihexanide in treatment of tissue loss wounds infected with Staphylococcuc aureus
Biography
Biography: Selvinaz Yakan
Abstract
The aim of this study is to present the effects on wound healing of Hypericum perforatum and polihexanide use in tissue loss
wounds infected with Staphylococcuc aureus. The research was carried out on 48 adult female Wistar albino rats. Under general
anaesthesia, wounds were created with 1 cm skin incission on the back of all rats. After 24 hours, the injection was made to wound
with Staphylococcuc aureus suspension. After 48 hours, wound cultures were obtained for each rats. At Çukurova University Hospital,
Department of Microbiology, inoculation was performed instead of appropriate fattening. This procedure was repeated every 48
hours until culture results are obtained in reproduction. As soon as reproduction was detected in cultures, treatment began. Rats
were randomly separated in to 4 main groups each group consisted of 12 animals. Control group (burned but no topical agent), in the
Hypericum perforatum oil groups, 1 mL of Hypericum perforatum oil was administered via syringe to the wound, in the polihexanide
groups, 1 mL of polihexanid was administered via syringe to the wound, in the Hypericum perforatum oil and polihexanid groups
in which both Hypericum perforatum and polihexanide were applied a total of 1 mL was administered via syringe to the wound.
The changes in the diameter of the wounds and the clinical course were evaluated daily. Tissue samples were taken by punch biopsy
under anaesthesia at the 7th and 14th day and histopathological evaluations were obtained. This study showed that both Hypericum
perforatum and polihexanid are effective in treatment of tissue loss wounds infected with Staphylococcuc aureus in rats.