9^th Global Veterinary Summit

In the last decades adverse food reactions were considerably increased in dogs and cats. We reported the onset of food intolerances

symptoms, including otitis, diarrhea, generalized anxiety, and dermatitis, in a cohort of 8 dogs consuming commercial diets. The

aim of this preliminary study is to possibly provide new insights in the idiopathic food intolerances with a particular focus on the

role of or their metabolites as possible triggering factors. All dogs were supplemented with an organic chicken-based diet for 15

days. Blood biochemical parameters, kibbles composition, oxytetracycline (OTC) serum concentration were performed before and after 15 days of organic chicken-based diet supplementation. We hypothesize that a chronic intake of contaminated food, enhanced

also by the presence of nanoparticles, might foster pharmacologic or idiopathic food intolerances. Biochemical analyses indicated a

significant increase in the alkaline phosphatase, from 41 to 52.5 U/L, after 15 days (**p<0.01), while a significant decrease in Gammaglutamyl transferase and urea, from 9.37 to 6.25 U/L and from 32.13±8.72 to 22.13±7.8 mg/dL, respectively, was observed (*p<0.05).A significant decrease in mean OTC serum concentration was also observed from 0.22 to 0.02 μg/mL (**p<0.01). We provided

clinical evidence of the presence of symptoms, which may occur in dogs fed on commercially available pet food diets. Nevertheless

we cannot rule out the possible combined pro-inflammatory activity exerted by nanoparticles, whose presence is almost unavoidable

during the overall production process.