Ali Shabestari Asl
Islamic Azad University
Iran
Title: Scanning electron microscopy assessment of canine gastric Helicobacter spp. in naturally infected dogs
Biography
Biography: Ali Shabestari Asl
Abstract
Different gastric Helicobacter like organisms was reported from dogs’ stomach but it is not known whether these species represent all the canine gastric helicobacters or some of them are more common. The aim of this study was morphological evaluation of canine gastric helicobacter spp. in fresh gastric samples of naturally infected dogs by electron microscopy. Thirty two stomachs of stray dogs were evaluated. Four gastric specimens were taken from all stomachs and used for rapid urease test (RUT), light microscopy, scanning electron microscopy (SEM) and polymerase chain reaction (PCR) examinations. Light microscopy confirmed the presence of gastric helicobacter like organisms in 29 of 32 stray dogs (90.5%). 87.5% and 94% of gastric samples were positive in RUT and PCR tests respectively. Four distinguishable canine gastric helicobacter organisms were confirmed by SEM investigation. Three strains of these organisms were established as H. felis candidatus, H. heilmanii and H. bizzozeronii because of their apparent morphological differences and PCR results. One strain of these bacteria was not distinguishable with routine studies. To confirmation of different types of canine gastric helicobacters, large scale studies with fast, simple recognition methods are recommended. This study portrays the need for further investigations in canine GHLO’s because some atypical canine gastric helicobacters were seen in canine stomachs.