Sayed Ahmed M
Jazan University
Saudi Arabia
Title: Molecular and epidemiological investigation of Brucellosis in Sharqyia, Egypt
Biography
Biography: Sayed Ahmed M
Abstract
The aim of the present study was to evaluate of the vaccination program and immune response of vaccinated animals in Sharqyia province. The study was applied on 4772 lactating and non-lactating survey the prevalence of brucellosis from 2008 to 2011. Sera were collected from animals during routine diagnosis and control program. Brucella serological tests namely Buffered Acidified Plate Antigen (BAPA cows of some private farms and individual distributed animals on different districts in Sharqyia province to Rose Bengal Plate (RBP), Tube Agglutination (TAT) and Rivanol test (Riv.T), Complement fixation test (CFT) and enzyme linked immunosorbent assay (ELISA) were applied. Furthermore, milk and tissues especially lymph nodes were collected from some reactor animals for bacteriological isolation and identification of the causative microorganism. Brucella was isolated using polymerase chain reaction (RT-PCR). Our results revealed that the prevalence of Brucella were 3.06% of private farms compared to 2.82% for individual animals. Bacteriological examination revealed that Brucella melitensis biovar-3 was isolated. These reflect the role of cattle in transmission and spreading of Brucella.