Global Veterinary Summit

Biography

Biography: Talib A Jaayid

Abstract

This study was carried out at genetic engineering lab, Agriculture College, University of Basrah (UoB). One hundred samples were analyzed from Iraqi camel belonged to six provinces: Basrah, Maysan, Qadisiyah, Najaf, Dhi Qar and Muthanna. Twelve microsatellite markers named CMS50, CMS121, CMS13, VOLP67, LCA66, CVRL06, CVRL05, CVRL01, VOLP32, VOLP03, WYLL44 and WYLL08 were used. All molecular markers amplified the camel DNA, produced a PCR product and generated novel and polymorphic DNA fragments in all tested samples with the exception of YWLL08 and YWLL04 which filled to produce amplifiable DNA. The total numbers of alleles for all markers were 119 alleles with an average of 11.9 alleles. VOLP67 marker had higher genetic polymorphism as produced 23 alleles size ranged from 161-244 bp. Observed number of alleles “No” ranged from 16 alleles in Najaf for VOLP67 marker to 4 alleles in Muthanna for CVRL06 marker. The observed heterozygosity “Ho” was 1.0 and the expected heterozygosity “He” was 0.936 in Maysan for VOLP67 marker while 0.682 in Dhi Qar for CVRL06 marker. Effective numbers of alleles “Ne” ranged from 2,941 in Dhi Qar for CVRL06 marker to 10.958 in Najaf for VOLP67 marker. Polymorphism information content PIC ranged from 0.595 in Dhi Qar for CVRL06 marker to 0.919 in Qadisiyah for CMS13 marker. The lower genetic distance and higher genetic similarity between Basrah and Najaf were 0.161 and 0.851 respectively while higher genetic distance and lower genetic similarity between Maysan and Dhi Qar populations were 0.370 and 0.690 respectively.